What is the purpose of buffy coat?

The buffy coat contains white blood cells, the soldiers of the immune system. At SBC, we provide buffy coats for research purposes – primarily used to study immune response and other white blood cell functions.

What is the function of the buffy coat?

A buffy coat contains leukocytes in a concentrated suspension, originating from whole blood or bone marrow. Generating a buffy coat from whole blood samples helps to concentrate large sample volumes and reduce downstream cell separation handling.

What does a thick buffy coat indicate?

The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8% of patients whose conventional thick films show negative parasitemia.

What is the buffy coat method?

In order to optimize and facilitate the diagnostics of blood parasites, a concentration technique was developed for application in parasitology research, the buffy coat method (BCM). The method is based on blood centrifugation and the resulting separation of blood cells and parasites in different layers [45].

What does the buffy coat separate?

BUFFY COAT – a cellular fraction containing platelets and white blood cells obtained from centrifugation of anticoagulated whole blood, which separates the blood components by density.

What does a thick buffy coat indicate?

The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8% of patients whose conventional thick films show negative parasitemia.

What is the buffy coat method?

In order to optimize and facilitate the diagnostics of blood parasites, a concentration technique was developed for application in parasitology research, the buffy coat method (BCM). The method is based on blood centrifugation and the resulting separation of blood cells and parasites in different layers [45].

Why is it called the buffy coat?

When researchers put the sample through a centrifuge, a machine that spins the blood, those WBCs and platelets combine to form their own layer suspended between the red blood cells (RBCs) and supernatant plasma. This thin layer is called a buffy coat because of its color (yellowish to brownish).

What percentage of blood is buffy coat?

What is the Buffy Coat? The buffy coat accounts for less than 1 percent of a whole blood sample, yet it contains the majority of white blood cells (WBC) and platelets.

What is the buffy coat method?

In order to optimize and facilitate the diagnostics of blood parasites, a concentration technique was developed for application in parasitology research, the buffy coat method (BCM). The method is based on blood centrifugation and the resulting separation of blood cells and parasites in different layers [45].

What does a thick buffy coat indicate?

The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8% of patients whose conventional thick films show negative parasitemia.

Watch What is the purpose of buffy coat Video